Article Text

PDF
Glutathione peroxidase activity and expression levels are significantly increased in acute coronary syndromes
  1. Ana Holley1,2,
  2. Janet Pitman1,
  3. John Miller1,2,
  4. Scott Harding1,2,3,
  5. Peter Larsen1,2,4
  1. 1School of Biological Sciences, Victoria University of Wellington, Wellington, New Zealand
  2. 2Wellington Cardiovascular Research Group, Wellington, New Zealand
  3. 3Department of Cardiology, Wellington Hospital, Wellington, New Zealand
  4. 4Department of Surgery and Anaesthesia, Otago University, Wellington, New Zealand
  1. Correspondence to Dr Ana Holley, Clinical Research Laboratory, Victoria University of Wellington, Level 8, Clinical Services Block, Wellington Hospital, Wellington 6021, New Zealand; ana_holley{at}hotmail.com

Abstract

High levels of the antioxidant enzyme, glutathione peroxidase (GPx), have been associated with improved outcomes following acute coronary syndromes (ACS), suggesting a protective role. How GPx levels are altered with coronary disease is not clearly established. This study examined GPx activity, protein, and mRNA levels in healthy controls, patients with stable coronary artery disease (CAD), and patients with ACS. We studied 20 individuals from each of the healthy control, stable CAD, and ACS groups. GPx activity and protein levels, along with oxidized low-density lipoprotein (oxLDL) were assayed in plasma. GPx mRNA levels from whole blood were quantified using real-time PCR. Levels of GPx activity in the plasma were higher in ACS (109±7.7 U/mL) compared with patients with stable CAD (95.2±16.4 U/mL, p<0.01) and healthy controls (87.6±8.3 U/mL, p<0.001). Plasma GPx protein levels were also elevated in ACS (21.6±9.5 µg/mL) compared with patients with stable CAD (16.5±2.8 µg/mL, p<0.05) and healthy controls (16.3±5.3 µg/mL, p<0.05). Levels of GPX1, GPX3, and GPX4 mRNA were significantly higher in the patients with ACS. Levels of oxLDL were also significantly higher in patients with ACS (61.9±22.2 U/L) than in patients with stable CAD (47.8±10.4 U/L, p<0.05) and healthy controls (48.9±11.9 U/L, p<0.05). Levels of oxLDL, GPx activity, protein, and mRNA are all significantly higher in patients with ACS compared with patients with stable CAD and healthy controls. These findings suggest that GPx may be upregulated in response to a change in oxidative stress during an ACS.

  • Antioxidants
  • Clinical Research
  • Coronary Artery Disease

Statistics from Altmetric.com

Footnotes

  • Contributors All authors contributed significantly to the submitted work and have read and approved the submission of the manuscript.

  • Funding AH was supported by the Victoria University Doctoral Scholarship.

  • Competing interests None declared.

  • Ethics approval New Zealand Health and Disability Ethics Committee.

  • Provenance and peer review Not commissioned; externally peer reviewed.

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.